RT info:eu-repo/semantics/article
T1 CD84 Functions as a homophilic adhesion molecule and enhances IFN- g secretion:adhesion is mediated by Ig-like domain 1
A1 Martín, Margarita
A1 Romero, Xavier
A1 Fuente García, Miguel Ángel de la
A1 Tovar, Victoria
A1 Zapater, Nuria
A1 Esplugues, Enric
A1 Pizcueta, Pilar
A1 Bosch, Jaime
A1 Engel, Pablo
K1 Biología molecular
AB CD84 is a member of the CD2 subset of the Ig superfamily of cell surface molecules. Its cytoplasmic tail binds to Src homology 2 domain-containing protein 1A (signaling lymphocytic activation molecule-associated protein), a protein encoded by the X-linked lymphoproliferative disease gene. It is preferentially expressed on B lymphocytes, monocytes, and platelets. We show that it is also expressed on thymocytes and T cells. CD84 was positive on CD4 CD8  thymocytes, and its expression decreased with cell maturation. It is expressed on mature T cells preferentially on CD45RO . To identify the CD84 ligand, we generated a solubleIg fusion protein containing the human CD84 extracellular domains (CD84-Ig). Because receptor-ligand interactions occur between several members of this subfamily, we assayed CD84-Ig binding with all members of the CD2 family. CD84-Ig bound to CD84-transfected cells, whereas no binding was detected with cells expressing other CD2 subfamily receptors, showing that CD84 binds to itself. Anti-CD84 mAbs recognizing epitopes wholly within domain 1 of CD84 blocked the binding of the CD84-Ig fusion protein to CD84-transfected cells and platelets. Data from CD84 domain human/mouse chimeras further revealed that only the first extracellular domain of the molecule is involved in the ligand receptor recognition. The CD84-CD84 interaction was independent of its cytoplasmic tail. Finally, concurrent ligation of human CD84 with mAbs or CD84-Ig and CD3 enhanced IFN-secretion in human lymphocytes. Thus, CD84 is its own ligand and acts as a costimulatory molecule.
PB American Association of Immunologists
SN 0022-1767
YR 2001
FD 2001
LK http://uvadoc.uva.es/handle/10324/10152
UL http://uvadoc.uva.es/handle/10324/10152
LA eng
NO The Journal of Immunology 2001, 167(7): 3668–3676.
NO Producción Científica
DS UVaDOC
RD 22-nov-2024