RT info:eu-repo/semantics/article
T1 Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9
A1 Fuente García, Miguel Ángel de la
A1 Tovar, Victoria
A1 Villamor, Neus
A1 Zapater, Nuria
A1 Pizcueta, Pilar
A1 Campo, Elias
A1 Jaime Bosch, Jaime
A1 Engel, Pablo
K1 Hematología
AB .Ly-9 is a mouse cell-surface glycoprotein that is selectively expressed on thymocytes and on mature T and B lymphocytes. Ly-9 belongs to the CD2 subset of the immunoglobulin superfamily, an emerging family of cell signaling receptors. Recently, a partial human Ly-9 complementary DNA (cDNA) sequence has been described. Full-length cDNA clones were isolated that included the initiation codon, the sequence encoding the full signal peptide, and 14 amino acids more in the cytoplasmic domain than in the previously reported clone. The predicted extracellular domain of human Ly-9 contains 4 immunoglobulinlike domains, similar to those in mouse Ly-9. Northern blot analysis revealed that the human Ly-9 messenger RNA (2.6 kb) is expressed predominantly in lymph node, spleen, thymus, and peripheral blood leukocytes. Four monoclonal antibodies (mAbs) were raised against human Ly-9 by immunizing mice with the pre-B-cell line 300.19 stably transfected with human Ly-9 full-length cDNA. These mAbs strongly stained the surfaces of cells transfected with human Ly-9 cDNA but not of untransfected cells. Human Ly-9 expression was restricted to T and B lymphocytes and thymocytes, with the highest levels of expression on CD4(+)CD8(-) and CD4(-)CD8(+) thymocytes. Monocytes, granulocytes, platelets, and red blood cells were uniformly negative for Ly-9. These mAbs immunoprecipitated major polypeptides of 120 kd from the transfected cells and 120 kd and 100 kd from B-cell line Daudi, probably because of the cell-surface-expressed isoforms. These data demonstrate that human Ly-9 is a new marker for the study of normal and malignant leukocytes
PB American Society of Hematology
SN 0006-4971
YR 2001
FD 2001
LK http://uvadoc.uva.es/handle/10324/10360
UL http://uvadoc.uva.es/handle/10324/10360
LA eng
NO Blood, 2001, 97(11):3513-3520
NO Producción Científica
DS UVaDOC
RD 26-dic-2024