RT info:eu-repo/semantics/article
T1 CD148 is a membrane protein tyrosine phosphatase present in all hematopoietic lineages and is involved in signal transduction on lymphocytes
A1 Fuente García, Miguel Ángel de la
A1 Nicolás, Josep Maria
A1 Freed, John H.
A1 Palou, Eduard
A1 Thomas, Andrew P.
A1 Vilella, Ramón
A1 Vives, Jordi
A1 Gayá, Antoni
K1 Células hematopoyéticas
K1 Hematología
AB Evidence is presented showing that a protein tyrosine phosphatase different from CD45 is present on the membrane of human hematopoietic cells. The molecule recognized by the monoclonal antibody 143-41, which has been classified as CD148 in the VI International Workshop on Leukocyte Differentiation Antigens, was immunopurified and sequenced. The sequence obtained from N-terminus as well as from two different CNBr-digested peptides showed a close identity with a previously described tyrosine phosphatase named HPTP-eta/DEP-1. CD148 is present on all hematopoietic lineages, being expressed with higher intensity on granulocytes than on monocytes and lymphocytes. Interestingly, whereas it is clearly present on peripheral blood lymphocytes, it is poorly expressed on different lymphoid cell lines of T and B origin. When this protein tyrosine phosphatase was cocrosslinked with CD3, an inhibition of the normally observed calcium mobilization was observed. This inhibition correlates with a decrease in phospholipase C-gamma (PLC-gamma) phosphorylation and is similar to the one observed with CD45. In addition, it is shown that the crosslinking of the CD148 alone is also able to induce an increase in [Ca2+]i. This increase is abolished in the presence of genistein and by cocrosslinking with CD45. These data, together with the induction of tyrosine phosphorylation on several substrates, including PLC-gamma, after CD148 crosslinking, suggest the involvement of a tyrosine kinase-based signaling pathway in this process. In conclusion, the data presented show that CD148 corresponds to a previously described protein tyrosine phosphatase HPTP-eta/DEP-1 and that this molecule is involved in signal transduction in lymphocytes.
PB American Society of Hematology
SN 0006-4971
YR 1998
FD 1998
LK http://uvadoc.uva.es/handle/10324/10365
UL http://uvadoc.uva.es/handle/10324/10365
LA eng
NO Blood. 1998; 91(8):2800-2809
NO Producción Científica
DS UVaDOC
RD 27-dic-2024