RT info:eu-repo/semantics/article T1 CD84 leukocyte antigen is a new member of the Ig superfamily A1 Fuente García, Miguel Ángel de la A1 Pizcueta, Pilar A1 Nadal, Marga A1 Bosch, Jaime A1 Engel, Pablo K1 Hematología K1 Células hematopoyéticas AB cDNA isolated from a human B-cell line Raji library was ana- (CD48 and HumLy9) have been mapped. CD84 monoclonal lyzed and shown to encode the full-length cDNA sequence antibodies (MoAbs) were shown to react with cells transof a novel cell-surface glycoprotein, initially termed HLy9-b. fected with the cloned cDNA. These MoAbs were furtherThe predicted mature 307-amino acid protein was composed used to show that CD84 is expressed as a single chain cellof two extracellular Ig-like domains, a hydrophobic trans- surface glycoprotein of Mr 64,000 to 82,000, which wasmembrane region, and an 83-amino acid cytoplasmic do- highly glycosylated. CD84 had a unique pattern of expresmain.The extracellular Ig-like domains presented structural sion, being found predominantly on lymphocytes and monoand sequence homology with a group of members of the Ig cytes. Thus, the glycoprotein HLy9-b is recognized by MoAbs superfamily that included CD2, CD48, CD58, and Ly9. North- previously clustered as CD84 and represents a newly identiern blot analysis showed that the expression of HLy9-b was fied member of the Ig superfamily that may play a significant predominantly restricted to hematopoietic tissues. Chromo- role in leukocyte activation. PB American Society of Hematology SN 0006-4971 YR 1997 FD 1997 LK http://uvadoc.uva.es/handle/10324/10379 UL http://uvadoc.uva.es/handle/10324/10379 LA eng NO Blood,1997, Vol 90, No 6, pp 2398-2405 NO Producción Científica DS UVaDOC RD 29-mar-2024