RT info:eu-repo/semantics/article
T1 CD50 (Intercellular Adhesion Molecule 3) Stimulation induces calcium mobilization and tyrmine phosphorylation through p59b and p56 in jurkat T cell line
A1 Juan, Manel
A1 Viñas, Odette
A1 Pino Otín, María Rosa
A1 Places, Lourdes
A1 Martínez Cáceres, Eva
A1 Barceló, Juan J.
A1 Miralles, Agustí
A1 Vilella, Ramón
A1 Fuente García, Miguel Ángel de la
A1 Vives, Jordi
A1 Yagüe, Jordi
A1 Gayá, Antoni
K1 Biología celular
AB The leukocyte differentiation antigen, CD50, has been recently identified as the intercellular adhesion molecule 3 (ICAM-3), the third counter-receptor of leukocyte function-associated antigen 1 (LFA-1). This molecule seems to be specially involved in the adhesion events of the initial phases of the immune response. To characterize the role of CD50 in leukocyte interactions, the different molecular events induced after cross-linking of CD50 on T cell-derived Jurkat cell line have been analyzed. When cells were incubated with anti-CD50 mAbs and cross-linked with polyclonal goat anti-mouse immunoglobulins, a rise in intracellular calcium concentration ([Ca2+]i) was observed. This increase in [Ca2+]i was mainly due to the uptake of extracellular Ca2+. This Ca2+ flux involved tyrosine phosphorylations and was further increased by CD3 costimulation. These data, together with those obtained by phosphotyrosine (P-Tyr) immunoprecipitation and in vitro kinase assays, suggested the involvement of protein-tyrosine kinases (PTK) in CD50 transduction pathways. By using specific antisera, the presence of p56lck and p59fyn protein tyrosine kinases (PTK) was clearly demonstrated in the CD50 immunoprecipitates. These findings suggest that the interaction of CD50 with its natural ligand (LFA-1) may result in T lymphocyte activation events, in which CD50 could play a very active role after antigen triggering.
PB Rockefeller University Press
SN 0022-1007
YR 1994
FD 1994
LK http://uvadoc.uva.es/handle/10324/10386
UL http://uvadoc.uva.es/handle/10324/10386
LA eng
NO Journal of Experimental Medicine, 1994, vol. 179, n. 6. p. 1747-1756
NO Producción Científica
DS UVaDOC
RD 22-nov-2024