RT info:eu-repo/semantics/article
T1 Addition of insulin-like growth factors (IGF-1) for the regeneration of critical defects in peripheral nerve injuries. Biological findings
A1 García Medrano, Belén
A1 Simón Pérez, Clarisa
A1 Barrio Sanz, Patricia
A1 Garrosa García, Manuel
A1 Gayoso Rodríguez, Manuel José
A1 Martín Ferrero, Miguel Ángel
K1 Regeneración celular
K1 Insulina
AB The purpose is analyze the biological and cellular regeneration of non-repairable lesions of peripheral nerve by muscle graft enhanced with growth factors.Methods: Controlled clinical experimental trial, level I evidence. Critical peripheral nerve injuries repair through acellular muscle graft alone versus its enrichment with IGF-1 (10 mg/ml mecasermin). Clinical and functional control for 3 months. Animals were sacrificed at 90-100 days, obtaining samples for macro and microscopy with blue toluidine staining, hematoxylin-eosin and Masson trichrome. Results: Regenerating axons can pass through a defect length of 15 mm with the addition of an acellular skeletal muscle graft and repopulate the distal nerve. Basal lamina tubes, acting as matrices for the axonal growth, appears that sensory recovery exceeds the rate of motor nerve fibers growth. Finding a number of regenerating axons greater than healthy sciatic, ensures the functional connection of distal axons to their target innervation. In contrast, grafts myelinated fibers are smaller than those of healthy sciatic nerve.Discussion: Acellular muscle fibers basal lamina define tubes that could support axonal regeneration, acting as matrices for the growth of axons, when grafted into a nerve defect. Stereologic studies biological sections to quantify the increase in the total number of axons, the density thereof and the thickness of the myelin from microscopic examination of histological slices of animals treated with the growth factor IGF-1.
PB OMICS Publishing Group
SN 2157-7099
YR 2014
FD 2014
LK http://uvadoc.uva.es/handle/10324/10637
UL http://uvadoc.uva.es/handle/10324/10637
LA eng
NO J Cytol Histol 2014, 5(2): 221-226
NO Producción Científica
DS UVaDOC
RD 11-jul-2024