RT info:eu-repo/semantics/bookPart T1 Calcium Imaging in Neuron Cell Death A1 Calvo Rodríguez, María A1 Villalobos Jorge, Carlos A1 Núñez Llorente, Lucía K1 Calcium imaging K1 Neuron death K1 Apoptosis K1 Hippocampal neurons K1 Fura2/AM AB Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuroncell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity.Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt) in neurons concurrentlywith apoptosis and neuron cell death is widely required.Procedures for culture and preparation of primary cultures of hippocampal rat neurons and fluorescenceimaging of cytosolic Ca2+ concentration in Fura2/AM-loaded neurons are described. We alsodescribe a method for apoptosis detection by immunofluorescence imaging. Finally, a simple method forconcurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described. PB Springer Science+Business Media New York SN 978-1-4939-2152-2 YR 2015 FD 2015 LK http://uvadoc.uva.es/handle/10324/21837 UL http://uvadoc.uva.es/handle/10324/21837 LA eng NO Lossi, Laura and Merighi, Adalberto, editors. Neuronal Cell Death: Methods and Protocols, Springer, 2015, p. 73 – 85 (Methods in Molecular Biology; 1254) NO Producción Científica DS UVaDOC RD 24-nov-2024