RT info:eu-repo/semantics/article
T1 Tissue reaction after intrastromal corneal ring implantation in an experimental animal model
A1 Ibares Frías, Lucía
A1 Gallego Muñoz, Patricia
A1 Cantalapiedra Rodríguez, Roberto
A1 Cruz Valsero, Maria Cruz
A1 Mar Sardaña, Santiago
A1 Merayo Lloves, Jesús
A1 Martínez García, María del Carmen
K1 Gallinas - Ensayos técnicos
K1 Córnea - Cirugía
AB Purpose To evaluate corneal wound healing in the hen animalmodel after additive surgery with an intracorneal ring segment(ICRS).Methods We implanted one ICRS in each eye of 76 hens. Incontrol group 1 (n=22 hens), the stromal channel was preparedbut no ICRS was inserted. In control group 2 (n=2hens), no surgery was performed. Animals were randomlyseparated into groups and euthanized after clinical follow-upof 4 and 12 hours, 1, 2, 3, and 7 days, and 1, 2, 3, 4, and6 months. Corneas were stained with hematoxylin-eosin. Apoptosiswas measured by terminal uridine nick end-labelingassays. Cell proliferation and myofibroblast-like differentiationwere assayed by BrdU and α-smooth muscle actin immunofluorescencemicroscopy. Stromal matrix changes weredocumented by electron microscopy.Results Epithelial and stromal cell apoptosis around theICRS-implanted and control group 1 eyes peaked at12 hours, but continued for 72 hours. In ICRSimplantedeyes, epithelial and stromal proliferation waspresent at 12 and 24 hours, respectively, and peaked at7 days and 72 hours, respectively. Some proliferation inthe ICRS-implanted group continued through the 6-month follow-up, and myofibroblast-like cells differentiatedone to three months after ICRS implantation. Thesegments rotated within the stroma as the limbal inferiorangle approached the epithelium.Conclusions Wound healing after ICRS implantation in hencorneas was similar to that of other corneal surgical wounds instages. However, there were some specific features related tothe small size of the epithelial wound and the device permanentlyimplanted inside the cornea.
PB Springer Verlag
SN 0721-832X
YR 2015
FD 2015
LK http://uvadoc.uva.es/handle/10324/22085
UL http://uvadoc.uva.es/handle/10324/22085
LA eng
NO Graefe's Archive for Clinical and Experimental Ophthalmology, (2015); 253:1071–1083
NO Producción Científica
DS UVaDOC
RD 22-nov-2024