RT info:eu-repo/semantics/article
T1 Human corneal fibroblast migration and ECM synthesis during stromal repair: Role played by PDGF-BB, bFGF, and TGFβ1 (HCFs migration and ECM synthesis during stromal repair: GFs effects)
A1 Gallego Muñoz, Patricia
A1 Ibares Frías, Lucía
A1 Garrote Adrados, José Antonio
A1 Valsero Blanco, María Cruz
A1 Cantalapiedra Rodríguez, Roberto
A1 Merayo Lloves, Jesús
A1 Martínez García, María del Carmen
K1 Córnea - Enfermedades
AB The development of treatments that modulate corneal wound healing to avoid fibrosis during tissue repair is important for the restoration of corneal transparency after an injury. To date, few studies have studied the influence of growth factors (GFs) on human corneal fibroblast (HCF) expression of extracellular matrix (ECM) proteins such as collagen types I and III, proteoglycans such as perlecan, or proteins implicated in cellular migration such as α5β1-integrin and syndecan-4. Using in vitro HCFs, we developed a mechanical wound model to study the influence of the GFs basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB), and transforming growth factor beta 1 (TGFβ1) on ECM protein production and cellular migration. Our results show that mechanical wounding provokes the autocrine release of bFGF and TGFβ1 at different time points during the wound closure. The HCF response to PDGF-BB was a rapid closure due to fast cellular migration associated with a high focal adhesion replacement and a high expression of collagen and proteoglycans, producing a non-fibrotic healing. bFGF stimulated non-fibrotic ECM production and limited the migration process. Finally, TGFβ1 induced expression of the fibrotic markers collagen type III and α5β1 integrin, and it inhibited cellular migration due to the formation of focal adhesions with a low turnover rate. The novel in vitro HCF mechanical wound model can be used to understand the role played by GFs in human corneal repair. The model can also be used to test the effects of different treatments aimed at improving the healing process.
PB Wiley
SN 1932-6254
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/22096
UL http://uvadoc.uva.es/handle/10324/22096
LA eng
NO J Tissue Eng Regen Med. 2016 Nov 15
NO Producción Científica
DS UVaDOC
RD 07-ago-2024