RT info:eu-repo/semantics/article
T1 Elastin-like recombinamers with acquired functionalities for gene-delivery applications
A1 Piña Lancho, María Jesús
A1 Susan, M. Alex
A1 Arias Vallejo, Francisco Javier
A1 Santos García, María Mercedes
A1 Rodríguez Cabello, José Carlos
A1 Mannemcherril, Rehka R
A1 Sharma, Chandra P.
K1 ELR
K1 Elastin-like Recombinamers
K1 Gene therapy
K1 Cationic Polymers
K1 Polyplex
AB In this work, well-defined elastin-like recombinamers (ELRs) were studied as a choice to the existing nonviral vectors due to their biocompatibility and ease of scale-up. Functional motifs, namely penetratin and LAEL fusogenic peptides were incorporated into a basic ELR sequence, and imidazole groups were subsequently covalently bound obtaining ELRs with new functionalities. Stable polyplexes composed of plasmid DNA and ELRs were formed. A particle size around 200 nm and a zeta potential up to nearly +24 mV made them suitable for gene delivery purposes. Additionally, viability and transfection assays with C6 rat glioma cell line showed an increase in the cellular uptake and transfection levels for the construction containing the LAEL motif. This study highlights the importance of controlling the polymer functionality using recombinant techniques and establishes the utility of ELRs as biocompatible nonviral systems for gene-therapy applications.
PB Society for Biomaterials
YR 2015
FD 2015
LK http://uvadoc.uva.es/handle/10324/22962
UL http://uvadoc.uva.es/handle/10324/22962
LA eng
NO J Biomed Mater Res A. 2015 Oct;103(10):3166-78
NO Producción Científica
DS UVaDOC
RD 25-nov-2024