RT info:eu-repo/semantics/article
T1 Anti-human endoglin (hCD105) immunotoxin--containing recombinant single chain ribosome-inactivating protein musarmin 1
A1 Barriuso, Begoña
A1 Jiménez López, María del Pilar
A1 Córdoba Diaz, Manuel
A1 Córdoba Diaz, Damián
A1 Girbés Juan, Tomás
A1 Arias Vallejo, Francisco Javier
A1 Girotti, Alessandra
K1 Cancer
K1 Cáncer
K1 Anti-tumor therapy
K1 Terapia antitumoral
AB Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT)—containing recombinant musarmin 1 (single chain ribosome-inactivating proteins) linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 × 10−10 to 10−9 M.
PB MDPI
SN 2072-6651
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/24415
UL http://uvadoc.uva.es/handle/10324/24415
LA eng
NO Toxins, 2016, vol. 8, n. 6.  p.
NO Producción Científica
DS UVaDOC
RD 22-nov-2024