RT info:eu-repo/semantics/article
T1 Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel
A1 Staubli, Sebastian Manuel
A1 Cerino, Giulia
A1 González de Torre, Israel
A1 Alonso Rodrigo, Matilde
A1 Oertli, Daniel
A1 Eckstein, Friedrich
A1 Glatz, Katharina
A1 Rodríguez Cabello, José Carlos
A1 Marsano, Anna
K1 Angiogénesis
AB The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELR)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we hypothesized that varying the cell adhesion properties of ELR-hydrogels could modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells. Human SVF cells, embedded in RGD-REDV-bioactivated or unmodified ELR-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusion-based culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized SVF-based hydrogels up to 28 days. ELR-based hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.
PB Elsevier
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/24758
UL http://uvadoc.uva.es/handle/10324/24758
LA eng
NO Biomaterials Volume 135, August 2017, Pages 30-41
NO Producción Científica
DS UVaDOC
RD 19-abr-2024