RT info:eu-repo/semantics/article
T1 Gelatin-Hyaluronic Acid Hydrogels with Tuned Stiffness to Counterbalance Cellular Forces and Promote Cell Differentiation
A1 Poveda Reyes, Sara
A1 Moulisova, Vladimira
A1 Sanmartín Masiá, Esther
A1 Quintanilla Sierra, Luis
A1 Salmerón Sánchez, Manuel
A1 Gallego Ferrer, Gloria
K1 Gelatina
K1 Ácido hialurónico
AB Cells interact mechanically with their environment, exerting mechanical forces that probe the extracellular matrix (ECM). The mechanical properties of the ECM determine cell behavior and control cell differentiation both in 2D and 3D environments. Gelatin (Gel) is a soft hydrogel into which cells can be embedded. This study shows significant 3D Gel shrinking due to the high traction cellular forces exerted by the cells on the matrix, which prevents cell differentiation. To modulate this process, Gel with hyaluronic acid (HA) has been combined in an injectable crosslinked hydrogel with controlled Gel–HA ratio. HA increases matrix stiffness. The addition of small amounts of HA leads to a significant reduction in hydrogel shrinking after cell encapsulation (C2C12 myoblasts). We show that hydrogel stiffness counterbalanced traction forces of cells and this was decisive in promoting cell differentiation and myotube formation of C2C12 encapsulated in the hybrid hydrogels.
PB Wiley
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/24816
UL http://uvadoc.uva.es/handle/10324/24816
LA eng
NO Macromolecular Bioscience, 2016, 16(9), pg. 1311-24
NO Producción Científica
DS UVaDOC
RD 16-jul-2024