RT info:eu-repo/semantics/article
T1 Viral gene transfer of dominant-negative Kv4 construct suppresses an O2-sensitive K+ current in chemoreceptor cells
A1 Pérez García, María Teresa
A1 López López, José Ramón
A1 Riesco Fagundo, Armenia María
A1 Hoppe, Uta C.
A1 Marbán, Eduardo
A1 González, Constancio
A1 Johns, David C.
K1 Carotid body
K1 Cuerpo carotídeo
K1 Hypoxia
K1 Hipoxia
K1 Potassium channels
K1 Canales de potasio
AB Hypoxia initiates the neurosecretory response of the  carotid body (CB) by inhibiting one or more potassium channels in the chemoreceptor cells. Oxygen-sensitive K+ channels were first described in rabbit CB chemoreceptor cells, in which a transient outward K+ current was reported to be reversibly inhibited by hypoxia. Although progress has been made to characterize this current with electrophysiological and pharmacological tools, no attempts have been made to identify which Kv channel proteins are expressed in rabbit CB chemoreceptor cells and to determine+ with adenoviruses that enabled ecdysone-inducible expression of the dominant-negative constructs and reporter genes in poly- cistronic vectors. In voltage-clamp experiments, we found that, whereas adenoviral infections of chemoreceptor cells with Kv1.xDN did not modify the O -sensitive K+ current, infections with Kv4.xDN  suppressed  the  transient  outward  current  in a time-dependent manner, significantly depolarized the cells, and abolished the depolarization induced by hypoxia. Our work dem- onstrate that genes of the Shal K+  channels underlie the tran-+ their contribution to the native O2-sensitive K current. To probe sient outward, O2-sensitive, K current of rabbit CB chemore- the molecular identity of this current, we have used dominant-negative  constructs  to  block  the  expression  of  functional  Kv channels of the Shaker (Kv1.xDN) or the Shal (Kv4.xDN) subfam- ceptor  cells  and  that  this  current  contributes  to  the  celldepolarization in response to low pO2.+ ilies, because members of these two subfamilies contribute to Key  words:  O2-sensitive  K current;  viral  gene  transfer; the transient outward K+ currents in other preparations. Delivery of the constructs into chemoreceptor cells has been achieved
PB The Society of Neuroscience
SN 5689-5695
YR 2000
FD 2000
LK http://uvadoc.uva.es/handle/10324/25950
UL http://uvadoc.uva.es/handle/10324/25950
LA eng
NO Journal of Neuroscience, 2000, vol. 20, n. 15. p. 5689-5695
NO Producción Científica
DS UVaDOC
RD 22-dic-2024