RT info:eu-repo/semantics/article
T1 Biogas-based polyhydroxyalkanoates production by Methylocystis hirsuta: A step further in anaerobic digestion biorefineries
A1 López, Juan Carlos
A1 Arnáiz, Esther
A1 Merchán Catalina, Laura
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
K1 Biorrefinería
K1 Metano
K1 Bio-refinery
K1 Methane
AB The potential of biogas (with and without H2S) and volatile fatty acids (VFAs) to support microbial growth and accumulation of polyhydroxyalkanoates (PHAs) in type II methanotrophs was evaluated batchwise under aerobic conditions. Methylocystis hirsuta was able to grow on artificial biogas (70% CH4, 29.5% CO2, 0.5% H2S) and accumulate PHA up to 45 ± 1% (wt.%) under N-limited conditions. The presence of CO2 and H2S did not significantly influence the growth and PHA synthesis in M. hirsuta compared to control tests provided with pure CH4 at similar concentrations. Likewise, the addition of VFAs to the cultivation broth at initial concentrations of 100–200 mg L−1 did not hamper the growth of this strain on artificial biogas. Indeed, the addition of 10% extra carbon in the form of individual VFAs resulted in an increase in the maximum PHA yield and final PHA content up to 0.45–0.63 gPHA gSubstrate 1 and 48–54% (wt.%), respectively, at the expense of a higher energy demand. Valeric acid supplementation supported the highest 3-hydroxyvalerate content (13.5%) within the biocomposite. In this context, this study demonstrated for the first time that 3-hydroxyvalerate synthesis by M. hirsuta did not depend on CH4 assimilation.
PB Elsevier
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/30103
UL http://uvadoc.uva.es/handle/10324/30103
LA eng
NO Chemical Engineering Journal, Volume 333, 2018, Pages 529-536
NO Producción Científica
DS UVaDOC
RD 24-nov-2024