RT info:eu-repo/semantics/article
T1 Enzyme sensor based on carbon nanotubes/cobalt(II) phthalocyanine and tyrosinase used in pharmaceutical analysis
A1 Rodríguez Méndez, María Luz
A1 Apetrei, Irina Mirela
A1 Apetrei, Constantin
A1 Saja Sáez, José Antonio de
AB A multicomponent enzyme sensor was used to evaluate phenolic compounds in plant-based pharmaceutical formulations. Multiwall carbon nanotubes were used as carbonaceous material used for the electrode construction. Cobalt phthalocyanine was used as electron mediator and tyrosinase as biocatalyst. The enzyme sensor performance was analyzed by cyclic voltammetry and amperometry in model solution of catechol and catechin. The cyclic voltammograms and the amperometric curves show an intense cathodic peak depending on the concentration of thephenolic compound. The cathodic peak was attributed to the reduction of enzymatically produced o-quinone at the enzyme sensor surface. For the model phenolics analyzed, the kinetics of the enzymatic reaction fitted into a Michaelis–Menten type kinetics, as confirmed by the h parameter close to 1 obtained from the Hill's plot. The detection limits were in the range of 1.66–6.32 μM demonstrating good quality performances of the enzyme sensor. Pharmaceutical samples were analyzed with multi-component biocomposite enzyme sensor to evaluate its real feasibility in pharmaceutical analysis.
SN 0925-4005
YR 2013
FD 2013
LK http://uvadoc.uva.es/handle/10324/30699
UL http://uvadoc.uva.es/handle/10324/30699
LA eng
NO Sensors and Actuators B  vol. 177 p.  138-144
NO Producción Científica
DS UVaDOC
RD 09-nov-2024