RT info:eu-repo/semantics/article
T1 Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method
A1 Rodríguez Méndez, María Luz
A1 Apetrei, Constantin
A1 Saja Sáez, José Antonio de
A1 Zurro, Javier
AB Tyrosinase-based biosensors containing a phthalocyanine as electron mediator have been prepared by two different methods. In the first approach, the enzyme and the electron mediator have been immobilized in carbon paste electrodes. In the second method, they have been introduced in an arachidic acid Langmuir-Blodgett nanostructured film that provides a biomimetic environment. The sensing properties of non-nanostructured and nanostructured biosensors towards catechol, catechin and phenol have been analyzed and compared. The enzyme retains the biocatalytic properties in both matrixes. However, the nanostructured biomimetic films show higher values of maximum reaction rates and lowest apparent Michaelis-Menten constants. In both types of sensors, the sensitivity follows the decreasing order catechol > catechin > phenol. The detection limits observed are in the range of 1.8–5.4 μM for Langmuir-Blodgett biosensors and 8.19–8.57 μM for carbon paste biosensors. In summary, it has been demonstrated that the Langmuir-Blodgett films provide a biomimetic environment and nanostructured biosensors show better performances in terms of kinetic, detection limit and stability.
SN 2073-4344
YR 2012
FD 2012
LK http://uvadoc.uva.es/handle/10324/30702
UL http://uvadoc.uva.es/handle/10324/30702
LA eng
NO Catalysts vol. 2 p. 517-531
DS UVaDOC
RD 22-dic-2024