RT info:eu-repo/semantics/article
T1 Single-cell phenotypic characterization of human pituitary GHomas and non-functioning adenomas based on hormone content and calcium responses to hypothalamic releasing hormones
A1 Senovilla González, Laura
A1 Núñez Llorente, Lucía
A1 Campos, José María de
A1 Luis Román, Daniel Antonio de
A1 Romero Bobillo, Enrique
A1 García-Sancho Martín, Francisco Javier
A1 Villalobos Jorge, Carlos
K1 Hormonas liberadoras hipotalámicas
K1 Hypothalamic releasing hormones
AB Human pituitary tumors are generally benign adenomas causing considerable morbidity due to excess hormone secretion, hypopituitarism, and other tumor mass effects. Pituitary tumors are highly heterogeneous and difficult to type, often containing mixed cell phenotypes. We have used calcium imaging followed by multiple immunocytochemistry to type growth hormone secreting (GHomas) and non-functioning pituitary adenomas (NFPAs). Individual cells were typed for stored hormones and calcium responses to classic hypothalamic releasing hormones (HRHs). We found that GHomas contained growth hormone cells either lacking responses to HRHs or responding to all four HRHs. However, most GHoma cells were polyhormonal cells responsive to both thyrotropin-releasing hormone (TRH) and GH-releasing hormone. NFPAs were also highly heterogeneous. Some of them contained ACTH cells lacking responses to HRHs or polyhormonal gonadotropes responsive to LHRH and TRH. However, most NFPAs were made of cells storing no hormone and responded only to TRH. These results may provide new insights on the ontogeny of GHomas and NFPAs.
PB Frontiers Media
SN 2234-943X
YR 2015
FD 2015
LK http://uvadoc.uva.es/handle/10324/32538
UL http://uvadoc.uva.es/handle/10324/32538
LA eng
NO Frontiers in Oncology, 2015, 5:124
NO Producción Científica
DS UVaDOC
RD 22-dic-2024