RT info:eu-repo/semantics/bookPart
T1 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
A1 Alonso Alonso, María Teresa
A1 Navas Navarro, Paloma
A1 García-Sancho Martín, Francisco Javier
K1 Mitocondrias
K1 Calcio
K1 Mitochondrial Calcium
AB Mitochondrial Ca2+ homeostasis is crucial for regulating vital functions such as respiration or apoptosis. Targeted aequorins are excellent probes to measure subcellular Ca2+. Ca2+ concentration in mitochondria ([Ca2+]M) is low at rest (about 10−7 M) and can increase to the micromolar or even approach the millimolar range, upon cell activation. Here we describe a new quantitative luminescent protocol to directly measure mitochondrial Ca2+ uptake, optimized for high throughput. The sensitivity of the method allows detection of changes in either the capacity or the affinity of mitochondrial Ca2+ transport.
PB Humana Press
SN 978-1-4939-6824-4
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/32548
UL http://uvadoc.uva.es/handle/10324/32548
LA eng
NO Mokranjac Dejana, Perocchi Fabiana (eds). Mitochondria: Practical Protocols. Humana Press, New York, NY, 2017. Methods in Molecular Biology book series (MIMB, 1567)
NO Producción Científica
DS UVaDOC
RD 24-nov-2024