RT info:eu-repo/semantics/article T1 Bicyclic RGD peptides with high integrin αvβ3 and α5β1 affinity promote cell adhesion on elastin-like recombinamers A1 Cipriani, Filippo A1 Dominik Bernhagen A1 García Arévalo, María del Carmen A1 González de Torre, Israel A1 Timmerman, Peter A1 Rodríguez Cabello, José Carlos K1 Medicina regenerativa K1 Regenerative medicine AB Biomaterial design in tissue engineering aims to identify appropriate cellular microenvironments in which cells can grow and guide new tissue formation. Despite the large diversity of synthetic polymers available for regenerative medicine, most of them fail to fully match the functional properties of their native counterparts. In contrast, the few biological alternatives employed as biomaterials lack the versatility that chemical synthesis can offer. Herein, we studied the HUVEC adhesion and proliferation properties of elastin-like recombinamers (ELRs) that were covalently functionalized with each three high-affinity and selectivity αvβ3- and α5β1-binding bicyclic RGD peptides. Next to the bicycles, ELRs were also functionalized with various integrin-binding benchmark peptides, i.e. knottin-RGD, cyclo-[KRGDf] and GRGDS, allowing for better classification of the obtained results. Covalent functionalization with the RGD peptides, as validated by MALDI-TOF analysis, guarantees flexibility and minimal steric hindrance for interactions with cellular integrins. In addition to the covalently modified RGD-ELRs, we also synthesized another benchmark ELR comprising RGD as part of the backbone. HUVEC adhesion and proliferation analysis using the PicoGreen® assay revealed a higher short-term adhesion and proliferative capacity of cells on ELR surfaces functionalized with high affinity, integrin-binding bicyclic RGD-peptides compared with the ELRs containing RGD in the backbone. PB IOP Publishing SN 1748-6041 YR 2019 FD 2019 LK http://uvadoc.uva.es/handle/10324/34880 UL http://uvadoc.uva.es/handle/10324/34880 LA eng NO Biomedical Materials, 2019 (in press) NO Producción Científica DS UVaDOC RD 04-dic-2024