RT info:eu-repo/semantics/article
T1 Bicyclic RGD peptides with high integrin α<sub>v</sub>β<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> affinity promote cell adhesion on elastin-like recombinamers
A1 Cipriani, Filippo
A1 Dominik Bernhagen
A1 García Arévalo, María del Carmen
A1 González de Torre, Israel
A1 Timmerman, Peter
A1 Rodríguez Cabello, José Carlos
K1 Medicina regenerativa
K1 Regenerative medicine
AB Biomaterial design in tissue engineering aims to identify appropriate cellular microenvironments in which cells can grow and guide new tissue formation. Despite the large diversity of synthetic polymers available for regenerative medicine, most of them fail to fully match the functional properties of their native counterparts. In contrast, the few biological alternatives employed as biomaterials lack the versatility that chemical synthesis can offer. Herein, we studied the HUVEC adhesion and proliferation properties of elastin-like recombinamers (ELRs) that were covalently functionalized with each three high-affinity and selectivity αvβ3- and α5β1-binding bicyclic RGD peptides. Next to the bicycles, ELRs were also functionalized with various integrin-binding benchmark peptides, i.e. knottin-RGD, cyclo-[KRGDf] and GRGDS, allowing for better classification of the obtained results. Covalent functionalization with the RGD peptides, as validated by MALDI-TOF analysis, guarantees flexibility and minimal steric hindrance for interactions with cellular integrins. In addition to the covalently modified RGD-ELRs, we also synthesized another benchmark ELR comprising RGD as part of the backbone. HUVEC adhesion and proliferation analysis using the PicoGreen® assay revealed a higher short-term adhesion and proliferative capacity of cells on ELR surfaces functionalized with high affinity, integrin-binding bicyclic RGD-peptides compared with the ELRs containing RGD in the backbone.
PB IOP Publishing
SN 1748-6041
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/34880
UL http://uvadoc.uva.es/handle/10324/34880
LA eng
NO Biomedical Materials, 2019 (in press)
NO Producción Científica
DS UVaDOC
RD 04-dic-2024