RT info:eu-repo/semantics/article
T1 Cartilage Regeneration in Preannealed Silk Elastin-Like Co-Recombinamers Injectable Hydrogel Embedded with Mature Chondrocytes in an Ex Vivo Culture Platform
A1 Cipriani, Filippo
A1 Krüger, Melanie
A1 González de Torre, Israel
A1 Quintanilla Sierra, Luis
A1 Alonso Rodrigo, Matilde
A1 Kock, Linda
A1 Rodríguez Cabello, José Carlos
K1 Regeneración del cartílago
K1 Cartilage Regeneration
K1 Elastina
K1 Elastin
AB Tissue engineering for cartilage repair requires biomaterials that show rapid gelation and adequate mechanical properties. Although the use of hydrogel is the most promising biomaterial, it often lacks in rigidity and anchorage of cells when they are surrounded by synovial fluid while they are subjected to heavy loads. We developed and produced the Silk Elastin-Like co-Recombinamer (SELR), which contains both the physical interaction from elastin motifs and from silk motifs. In the first part of this work, we set up and optimized a preannealing treatment based on the evolution of silk motifs into β-sheet structures in order to fulfill the required mechanical properties of hydrogels for cartilage repair. The new preannealed SELRs (pA(EIS)2-(I5R)6) were characterized with the combination of several experimental techniques (CD, TEM, SEM, and rheology) to provide a deep insight into the material features. Finally, the regeneration properties of the pA(EIS)2-(I5R)6 hydrogel embedded with chondrocytes were evaluated. After 4 weeks of culturing in a standardized and representative ex vivo model, the biochemical and histological analysis revealed the production of glycosaminglycans and collagen. Moreover, the immunohistochemistry showed the absence of fibro-cartilage and the presence of hyaline cartilage. Hence, we conclude that the pA(EIS)2-(I5R)6 hydrogel presents improved mechanical properties while conserving the injectability, which leads to successful regeneration of hyaline cartilage in an ex vivo model.
PB American Chemical Society
SN 1525-7797
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/34908
UL http://uvadoc.uva.es/handle/10324/34908
LA eng
NO Biomacromolecules, 2018, 19 (11), pp 4333–4347
NO Producción Científica
DS UVaDOC
RD 22-nov-2024