RT info:eu-repo/semantics/article
T1 Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery
A1 Cantera Ruiz de Pellón, Sara
A1 Sánchez Andrea, Irene
A1 Sadornil Delgado, Lidia Jimena
A1 García Encina, Pedro Antonio
A1 Stams, Alfons J.M.
A1 Muñoz Torre, Raúl
K1 Metano
K1 Methane
AB Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment of methanotrophic consortia able to co-produce different ectoines during CH4 metabolism. Four different enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented with 3 and 6% NaCl, and using methane as the sole carbon and energy source. The highest ectoine accumulation (∼20 mg ectoine g biomass−1) was recorded in the two consortia enriched at 6% NaCl (Cow6 and Slu6). Moreover, hydroxyectoine was detected for the first time using methane as a feedstock in Cow6 and Slu6 (∼5 mg g biomass−1). The majority of the haloalkaliphilic bacteria identified by 16S rRNA community profiling in both consortia have not been previously described as methanotrophs. From these enrichments, two novel strains (representing novel species) capable of using methane as the sole carbon and energy source were isolated: Alishewanella sp. strain RM1 and Halomonas sp. strain PGE1. Halomonas sp. strain PGE1 showed higher ectoine yields (70–92 mg ectoine g biomass−1) than those previously described for other methanotrophs under continuous cultivation mode (∼37–70 mg ectoine g biomass−1). The results here obtained highlight the potential of isolating novel methanotrophs in order to boost the competitiveness of industrial CH4-based ectoine production.
PB Elsevier
SN 0301-4797
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/35163
UL http://uvadoc.uva.es/handle/10324/35163
LA eng
NO Journal of Environmental Management, 2019, Volume 231, Pages 1091-1099
NO Producción Científica
DS UVaDOC
RD 18-nov-2024