RT info:eu-repo/semantics/article
T1 Measuring Ca2 + inside intracellular organelles with luminescent and fluorescent aequorin-based sensors
A1 Alonso Alonso, María Teresa
A1 Rojo Ruiz, Jonathan
A1 Navas Navarro, Paloma
A1 Rodríguez Prados, Macarena
A1 García-Sancho Martín, Francisco Javier
K1 Aequorina
K1 Aequorin
AB GFP-Aequorin Protein (GAP) can be used to measure [Ca2 +] inside intracellular organelles, both by luminescence and by fluorescence. The low-affinity variant GAP3 is adequate for ratiometric imaging in the endoplasmic reticulum and Golgi apparatus, and it can be combined with conventional synthetic indicators for simultaneous measurements of cytosolic Ca2 +. GAP is bioorthogonal as it does not have mammalian homologues, and it is robust and functionally expressed in transgenic flies and mice, where it can be used for Ca2 + measurements ex vivo and in vivo to explore animal models of health and disease. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.
PB Elsevier
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/35202
UL http://uvadoc.uva.es/handle/10324/35202
LA eng
NO Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Volume 1864, Issue 6, 2017, Pages 894-899
NO Producción Científica
DS UVaDOC
RD 24-nov-2024