RT info:eu-repo/semantics/article
T1 Tethering QK peptide to enhance angiogenesis in elastin-like recombinamer (ELR) hydrogels
A1 Flora, Tatjana
A1 González de Torre, Israel
A1 Alonso Rodrigo, Matilde
A1 Rodríguez Cabello, José Carlos
K1 Péptido QK
K1 QK peptide
K1 Angiogénesis
K1 Angiogenesis
K1 Hidrogeles de elastámero recombinante
K1 Elastin-like recombinamer hydrogels
AB The development of new capillary networks in engineered constructs is essential for their survival and their integration with the host tissue. It has recently been demonstrated that ELR-based hydrogels encoding different bioactivities are able to modulate their interaction with the host after injection or implantation, as indicated by an increase in cell adhesion andthe ability to trigger vascularization processes. Accordingly, the aim of this study was to increase their angiogenic ability both in vitro and in vivo using a small VEGF mimetic peptide named QK, which was tethered chemically to ELR-based hydrogels containing cell-adhesion sequences in their backbone, such as REDV and RGD, as well as a proteolytic site(VGVAPG). In vitro studies were performed using a co-culture of endothelial and fibroblast cells encapsulated into the ELR-based hydrogels in order to determine cell proliferation after 21 days of culture, as well as the number of cell-cell interactions. It was found that although the presence of this peptide does not influence the morphological and rheologicalproperties of these hydrogels, it has an effect on cell behaviour, inducing an increase in cell proliferation and the formation of endothelial cell clusters. In vivo studies demonstrate that the QK peptide enhances the formation of prominent functional capillaries at three weeks post-injection, as confirmed by H&E staining and CD31 immunohistochemistry. The newly formed functional microvasculature ensures perfusion and connection with surrounding tissues. These results show that ELR-QK hydrogels increase capillary network formation and are thereforeattractive candidates for application in tissue regeneration, for example for the treatment of cardiovascular diseases such as myocardial infarction or ischemia.
PB Springer Link
SN 0957-4530
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/35327
UL http://uvadoc.uva.es/handle/10324/35327
LA eng
NO Journal of Materials Science: Materials in Medicine, 2019, vol. 30, n. 30. 12 p.
NO Producción Científica
DS UVaDOC
RD 27-dic-2024