RT info:eu-repo/semantics/article
T1 Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers
A1 Flora, Tatjana
A1 González de Torre, Israel
A1 Quintanilla Sierra, Luis
A1 Alonso Rodrigo, Matilde
A1 Rodríguez Cabello, José Carlos
K1 Control espacial
K1 Spatial control
K1 Adhesión celular
K1 Cell adhesion
K1 Biofuncionalización superficial
K1 Surface bio-functionalization
K1 Ablación láser
K1 Laser ablation
K1 Recombinantes tipo elastina
K1 Elastin-like recombinamers
AB A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out.After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof.Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties.
PB Elsevier
SN 0014-3057
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/35330
UL http://uvadoc.uva.es/handle/10324/35330
LA eng
NO European Polymer Journal, 2018, vol. 106. p. 19-29
NO Producción Científica
DS UVaDOC
RD 22-nov-2024