RT info:eu-repo/semantics/article
T1 Use of proteolytic sequences with different cleavage kinetics as a way to generate hydrogels with preprogrammed cell-infiltration patterns imparted over their given 3D spatial structure
A1 Flora, Tatjana
A1 González de Torre, Israel
A1 Alonso Rodrigo, Matilde
A1 Rodríguez Cabello, José Carlos
K1 Biomaterials
K1 Biomateriales
K1 Angiogenesis
K1 Angiogénesis
K1 Elastin-like recombinamers
K1 Recombinantes tipo elastina
K1 3D system
K1 Sistema 3D
AB Control over biodegradation processes is crucial to generate advanced functional structures with a more interactive and efficient role for biomedical applications. Herein, a simple, high-throughput approach is developed based on a 3D-structured system that allows a preprogramed spatial-temporal control over cell infiltration and biodegradation. The 3D-structured system is based on elastin-like recombinamers (ELRs) characterized by differences in the kinetics of their peptide cleavage and consists of a three-layer hydrogel disk comprising an internal layer containing a rapidly degrading component, with the external layers containing a slow-degrading ELR. This structure is intended to invert the conventional pattern of cell infiltration, which goes from the outside to the inside of the implant, to allow an anti-natural process in which infiltration takes place first in the internal layer and later progresses to the outer layers. Time-course in vivo studies proved this hypothesis, i.e. that it is possible to drive the infiltration of cells over time in a given 3D-structured implant in a controlled and predesigned way that is able to overcome the natural tendency of conventional cell infiltration. The results obtained herein open up the possibility of applying this concept to more complex systems with multiple biological functions.
PB IOP Publishing
SN 1758-5090
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/36422
UL http://uvadoc.uva.es/handle/10324/36422
LA eng
NO Biofabrication, 2019, vol. 11, n. 3. 16 p.
NO Producción Científica
DS UVaDOC
RD 23-nov-2024