RT info:eu-repo/semantics/article T1 Optimisation of the production of fermentable monosaccharides from algal biomass grown in photobioreactors treating wastewater A1 Martín Juarez, Judit A1 Vega Alegre, María del Sol A1 Riol Pastor, Elena A1 Muñoz Torre, Raúl A1 Bolado Rodríguez, Silvia K1 Enzymatic hydrolysis K1 Hidrólisis enzimática K1 Swine manure K1 Estiércol porcino K1 Chemical pretreatment K1 Pretratamiento químico K1 Lipids K1 Lípidos K1 Proteins K1 Proteinas AB Biomass grown in wastewater treatment photobioreactors is a cheap raw material with high contents of carbohydrates, proteins and lipids. This work studies the production of fermentable monosaccharides from three biomasses grown in piggery wastewater (P), domestic wastewater (W) and synthetic medium (S) by applying chemical pretreatment and enzymatic hydrolysis, using a Taguchi design. ANOVA identified temperature, chemical reagent type and chemical reagent concentration as significant operational parameters. However, the biomass concentration, pretreatment time, enzyme dosage and enzymatic hydrolysis time had no remarkable effect. The bacterial content of the biomass had no relevant impact on carbohydrate and protein solubilisation but had a remarkable effect on the degradation of the released carbohydrates (57, 60 and 37% for P, W and S), while also affecting lipid solubilisation. Pretreatment with HCl 2 M at 120 °C resulted the optimal conditions, achieving a monosaccharide recovery of 53, 59 and 80% for P, W and S biomasses, respectively. PB Elsevier SN 0960-8524 YR 2019 FD 2019 LK http://uvadoc.uva.es/handle/10324/37243 UL http://uvadoc.uva.es/handle/10324/37243 LA eng NO Bioresource Technology, 2019, vol. 281. p. 239-249 NO Producción Científica DS UVaDOC RD 25-nov-2024