RT info:eu-repo/semantics/article
T1 Therapeutic Effect of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Experimental Corneal Failure Due to Limbal Stem Cell Niche Damage
A1 Galindo de la Rosa, Sara
A1 Herreras Cantalapiedra, José María
A1 López Paniagua, Marina
A1 Rey, Esther
A1 De La Mata Sampedro, Ana
A1 Plata Cordero, María
A1 Calonge, Margarita
A1 Nieto Miguel, Teresa
AB Limbal stem cells are responsible for the continuous renewal of the corneal epithelium. The destruction or dysfunction of these stem cells or their niche induces limbal stem cell deficiency (LSCD) leading to visual loss, chronic pain, and inflammation of the ocular surface. To restore the ocular surface in cases of bilateral LSCD, an extraocular source of stem cells is needed to avoid dependence on allogeneic limbal stem cells that are difficult to obtain, isolate, and culture. The aim of this work was to test the tolerance and the efficacy of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) to regenerate the ocular surface in two experimental models of LSCD that closely resemble the different severity grades of the human pathology. hAT-MSCs transplanted to the ocular surface of the partial and total LSCD models developed in rabbits were well tolerated, migrated to inflamed tissues, reduced inflammation, and restrained the evolution of corneal neovascularization and corneal opacity. The expression profile of the corneal epithelial cell markers CK3 and E-cadherin, and the limbal epithelial cell markers CK15 and p63 was lost in the LSCD models, but was partially recovered after hAT-MSC transplantation. For the first time, we demonstrated that hAT-MSCs improves corneal and limbal epithelial phenotypes in animal LSCD models. These results support the potential use of hAT-MSCs as a novel treatment of ocular surface failure due to LSCD. hAT-MSCs represent an available, non-immunogenic source of stem cells that may provide therapeutic benefits in addition to reduce health care expenses.
PB Wiley-Blackwell. 111 River St, Hoboken 07030-5774, NJ, USA
SN 1066-5099
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/40341
UL http://uvadoc.uva.es/handle/10324/40341
LA eng
NO Stem Cells. 2017. 35 (10): 2160–2174
NO Producción Científica
DS UVaDOC
RD 27-abr-2024