RT info:eu-repo/semantics/article T1 Layer-by-layer biofabrication of coronary covered stents with clickable elastin-like recombinamers A1 Fernández Colino, Alicia A1 Wolf, Frederic A1 Moreira, Ricardo A1 Rütten, Stephan A1 Schmitz-Rode, Thomas A1 Rodríguez Cabello, José Carlos A1 Jockenhoevel, Stefan A1 Mela, Petra K1 Coronary stents K1 Stents coronarios K1 Elastin-like recombinamers K1 Recombinantes tipo elastina K1 Click chemistry K1 Química click K1 Hemocompatibility K1 Hemocompatibilidad AB Coronary artery disease is the leading cause of death around the world. Endovascular stenting is the preferred treatment option to restore blood flow in the coronary arteries due to the lower perioperative morbidity when compared with more invasive treatment options. However, stent failure is still a major clinical problem, and further technological solutions are required to improve the performance of current stents. Here, we developed coronary stents covered with elastin-like recombinamers (ELRs) by exploiting a layer-by-layer technique combined with catalyst-free click chemistry. The resulting ELR-covered stents were intact after an in vitro simulated implantation procedure by balloon dilatation, which evidenced the elastic performance of the membrane. Additionally, the stents were mechanically stable under high flow conditions, which is in agreement with the covalent and stable nature of the click chemistry crosslinking strategy exploited during the ELR-membrane manufacturing and the successful embedding of the stent. Minimal platelet adhesion was detected after blood exposure in a Chandler loop as shown by scanning electron microscopy. The seeding of human endothelial progenitor cells (EPCs) on the ELR-membranes resulted in a confluent endothelial layer. These results prove the potential of this strategy to develop an advanced generation of coronary stents, with a stable and bioactive elastin-like membrane to exclude the atherosclerotic plaque from the blood stream or to seal coronary perforations and aneurysms, while providing a luminal surface with minimal platelet adhesion and favouring endothelialization. PB Elsevier SN 0014-3057 YR 2019 FD 2019 LK http://uvadoc.uva.es/handle/10324/40371 UL http://uvadoc.uva.es/handle/10324/40371 LA eng NO European Polymer Journal, 2019, vol. 121. 20 p. NO Producción Científica DS UVaDOC RD 20-sep-2024