RT info:eu-repo/semantics/article
T1 Layer-by-layer biofabrication of coronary covered stents with clickable elastin-like recombinamers
A1 Fernández Colino, Alicia
A1 Wolf, Frederic
A1 Moreira, Ricardo
A1 Rütten, Stephan
A1 Schmitz-Rode, Thomas
A1 Rodríguez Cabello, José Carlos
A1 Jockenhoevel, Stefan
A1 Mela, Petra
K1 Coronary stents
K1 Stents coronarios
K1 Elastin-like recombinamers
K1 Recombinantes tipo elastina
K1 Click chemistry
K1 Química click
K1 Hemocompatibility
K1 Hemocompatibilidad
AB Coronary artery disease is the leading cause of death around the world. Endovascular stenting is the preferred treatment option to restore blood flow in the coronary arteries due to the lower perioperative morbidity when compared with more invasive treatment options. However, stent failure is still a major clinical problem, and further technological solutions are required to improve the performance of current stents. Here, we developed coronary stents covered with elastin-like recombinamers (ELRs) by exploiting a layer-by-layer technique combined with catalyst-free click chemistry. The resulting ELR-covered stents were intact after an in vitro simulated implantation procedure by balloon dilatation, which evidenced the elastic performance of the membrane. Additionally, the stents were mechanically stable under high flow conditions, which is in agreement with the covalent and stable nature of the click chemistry crosslinking strategy exploited during the ELR-membrane manufacturing and the successful embedding of the stent. Minimal platelet adhesion was detected after blood exposure in a Chandler loop as shown by scanning electron microscopy. The seeding of human endothelial progenitor cells (EPCs) on the ELR-membranes resulted in a confluent endothelial layer. These results prove the potential of this strategy to develop an advanced generation of coronary stents, with a stable and bioactive elastin-like membrane to exclude the atherosclerotic plaque from the blood stream or to seal coronary perforations and aneurysms, while providing a luminal surface with minimal platelet adhesion and favouring endothelialization.
PB Elsevier
SN 0014-3057
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/40371
UL http://uvadoc.uva.es/handle/10324/40371
LA eng
NO European Polymer Journal, 2019, vol. 121. 20 p.
NO Producción Científica
DS UVaDOC
RD 23-sep-2024