RT info:eu-repo/semantics/article
T1 Biomarkers in ocular chronic graft versus host disease: tear cytokine- and chemokine-based predictive model.
A1 Cocho, Lidia
A1 Fernández Martínez, Itziar
A1 Calonge, Margarita
A1 Martínez, Verónica
A1 González García, María Jesús
A1 Caballero, Dolores
A1 López Corral, Lucía
A1 García Vázquez, Carmen
A1 Vázquez, Lourdes
A1 Stern, Michael E.
A1 Enriquez De Salamanca Aladro, Amalia
K1 Biomarcadores
AB Purpose: To develop a tear molecule level-based predictive model based on a panel of tear cytokines and their correlation with clinical features in ocular chronic graft versus host disease (cGVHD).Methods: Twenty-two ocular cGVHD patients and 21 healthy subjects were evaluated in a controlled environmental research laboratory (CERLab). Clinical parameters were recorded, and tears were collected. Levels of 15 molecules (epidermal growth factor [EGF], IL receptor antagonist [IL-1Ra], IL-1β, IL-2, IL-6, IL-8/CXCL8, IL-10, IL-12p70, IL-17A, interferon inducible protein [IP]-10/CXCL10, IFN-γ, VEGF, TNF-α, eotaxin 1, and regulated on activation normal T cell expressed and secreted [RANTES]) were measured by multiplex-bead assay and correlated with clinical parameters. Logistic regression was used to develop a predictive model. Leave-one-out cross-validation was applied. Classification capacity was evaluated in a cohort of individuals with dry eye (DE) of other etiologies different from GVHD.Results: Epidermal growth factor and IP-10/CXCL10 levels were significantly decreased in ocular cGVHD, positively correlating with tear production and stability and negatively correlating with symptoms, hyperemia, and vital staining. Interleukin-1Ra, IL-8/CXCL8, and IL-10 were significantly increased in ocular cGVHD, and the first two correlated positively with symptoms, hyperemia, and ocular surface integrity while negatively correlating with tear production and stability. Predictive models were generated, and the best panel was based on IL-8/CXCL8 and IP-10/CXCL10 tear levels along with age and sex, with an area under the receiving operating curve of 0.9004, sensitivity of 86.36%, and specificity of 95.24%.Conclusions: A predictive model based on tear levels of IL-8/CXCL8 and IP-10/CXCL10 resulted in optimal sensitivity and specificity. These results add further knowledge to the search for potential biomarkers in this devastating ocular inflammatory disease.
PB ARVO JOURNALS
SN 1552-5783
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/40639
UL http://uvadoc.uva.es/handle/10324/40639
LA eng
NO Invest Ophthalmol Vis Sci. 2016 Feb;57(2):746-58
NO Producción Científica
DS UVaDOC
RD 18-nov-2024