RT info:eu-repo/semantics/article
T1 The Fusarium Circinatum gene Fcrho1, encoding a putative Rho1 GTPase, is involved in vegetative growth but dispensable for pathogenic development
A1 Muñoz Adalia, Emigdio Jordán
A1 Cañizares Ruiz, María del Carmen
A1 Fernández Fernández, María Mercedes
A1 Díez Casero, Julio Javier
A1 García Pedrajas, María Dolores
K1 Cell signalling
K1 Señalización celular
K1 Deletion mutants
K1 Mutantes por delección
K1 Pine pitch canker disease
K1 Chancro resinoso del pino
K1 3106 Ciencia Forestal
AB Fusarium circinatum is the causal agent of pine pitch canker (PPC), one of the mostdevastating forest diseases worldwide. This fungus causes severe damping-off in pine seedlings andgrowth reduction, wilting and the development of cankers in pine forests and plantations. A draft ofthe complete genome sequence of this phytopathogen was recently made available. This informationwas used to annotate in silico the gene Fcrho1 as a putative homolog of Rho1 GTPase genes. In thisstudy, we generated Fcrho1 deletion mutants in two F. circinatum wildtype strains isolated fromdamaged trees in northern Spain. For that, we used a modified version of the OSCAR methodology,an approach not previously used in F. circinatum that allows the generation of deletion constructsin a single cloning step. The conidiation and spore germination of the resulting deletion mutantswere not affected, neither the hyphal morphology. However, the mutant strains showed significantlyreduced growth in vitro and more foamy macroscopic hyphal morphology than their correspondingectopic and wildtype strains. Finally, an in vivo virulence assay showed that the reduced in vitrogrowth rate characteristic to the deletion mutants does not impact their pathogenicity.
PB MDPI
SN 1999-4907
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/40820
UL http://uvadoc.uva.es/handle/10324/40820
LA eng
NO Forests, 2018, vol. 9, n. 11. 12 p.
NO Producción Científica
DS UVaDOC
RD 18-abr-2024