RT info:eu-repo/semantics/article
T1 Characterization of small RNAs originating from mitoviruses infecting the conifer pathogen Fusarium circinatum
A1 Muñoz Adalia, Emigdio Jordán
A1 Díez Casero, Julio Javier
A1 Fernández Fernández, María Mercedes
A1 Hantula, Jarkko
A1 Vainio, Eeva J.
K1 Pine pitch canker
K1 Chancro resinoso del pino
K1 RNA sequencing
K1 Secuenciación de ARN
K1 Mycovirus
K1 Micovirus
K1 3106 Ciencia Forestal
AB Deep sequencing of small RNAs has proved efective in the diagnosis of mycovirus infections. In this study, the presenceof mycoviruses in ten isolates of the phytopathogenic fungus Fusarium circinatum was investigated by high-throughputsequencing (HTS) of small RNAs. The contigs resulting from de novo assembly of the reads were aligned to viral genomesequences. The presence of each mycovirus detected in the isolates was confrmed by RT-PCR analysis with four previouslydescribed primer pairs and seven new pairs designed on the basis of sequencing data. The fndings demonstrate the potentialuse of HTS for reconstructing previously identifed mitoviruses infecting F. circinatum.
PB Springer
SN 1432-8798
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/40837
UL http://uvadoc.uva.es/handle/10324/40837
LA eng
NO Archives of Virology, 2018, vol. 163. p. 1009-1018
NO Producción Científica
DS UVaDOC
RD 24-nov-2024