RT info:eu-repo/semantics/article
T1 Transferability of PCR-based diagnostic protocols: An international collaborative case study assessing protocols targeting the quarantine pine pathogen Fusarium circinatum
A1 Ioos, Renaud
A1 Aloi, Francesco
A1 Piškur, Barbara
A1 Guinet, Cécile
A1 Mullett, Martin
A1 Berbegal, Mónica
A1 Bragança, Helena
A1 Cacciola, Santa Olga
A1 Oskay, Funda
A1 Cornejo, Carolina
A1 Adamson, Kalev
A1 Douanla-Meli, Clovis
A1 Kačergius, Audrius
A1 Martínez Álvarez, Pablo
A1 Nowakowska, Justyna Anna
A1 Luchi, Nicola
A1 Vettraino, Anna Maria
A1 Ahumada, Rodrigo
A1 Pasquali, Matias
A1 Fourie, Gerda
A1 Kanetis, Loukas
A1 Alves, Artur
A1 Ghelardini, Luisa
A1 Dvořák, Miloň
A1 Sanz Ros, Antonio Vicente
A1 Díez Casero, Julio Javier
A1 Baskarathevan, Jeyaseelan
A1 Aguayo, Jaime
K1 Pine pitch canker
K1 Chancro resinoso del pino
K1 PCR-based tests
K1 Tests PCR
K1 Diagnóstico de enfermedad fungica
K1 Fungal infections - Diagnosis
K1 Enfermedades fúngicas - Diagnóstico
K1 3106 Ciencia Forestal
AB Fusarium circinatum is a harmful pathogenic fungus mostly attacking Pinus species and alsoPseudotsuga menziesii, causing cankers in trees of all ages, damping-of in seedlings, and mortality incuttings and mother plants for clonal production. This fungus is listed as a quarantine pest in severalparts of the world and the trade of potentially contaminated pine material such as cuttings, seedlings orseeds is restricted in order to prevent its spread to disease-free areas. Inspection of plant material oftenrelies on DNA testing and several conventional or real-time PCR based tests targeting F. circinatum areavailable in the literature. In this work, an international collaborative study joined 23 partners to assessthe transferability and the performance of nine molecular protocols, using a wide panel of DNA from 71representative strains of F. circinatum and related Fusarium species. Diagnostic sensitivity, specifcityand accuracy of the nine protocols all reached values >80%, and the diagnostic specifcity was the onlyparameter difering signifcantly between protocols.The rates of false positives and of false negativeswere computed and only the false positive rates difered signifcantly, ranging from 3.0% to 17.3%.Thediference between protocols for some ofthe performance values were mainly due to cross-reactionswith DNA from non-target species, which were either not tested or documented in the original articles.Considering that participating laboratories were free to use their own reagents and equipment, thisstudy demonstrated that the diagnostic protocols for F. circinatum were not easily transferable to endusers. More generally, our results suggest that the use of protocols using conventional or real-time PCRoutside their initial development and validation conditions should require careful characterization ofthe performance data priorto use under modifed conditions (i.e. reagents and equipment). Suggestionsto improve the transfer are proposed.
PB Springer Nature
SN 2045-2322
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/40839
UL http://uvadoc.uva.es/handle/10324/40839
LA eng
NO Scientific Reports, 2019, vol. 9. 17 p.
NO Producción Científica
DS UVaDOC
RD 24-nov-2024