RT info:eu-repo/semantics/article
T1 Effect of mycoviruses on the virulence of Fusarium circinatum and laccase activity
A1 Muñoz Adalia, Emigdio Jordan
A1 Flores Pacheco, Juan Asdrúbal
A1 Martínez Álvarez, Pablo
A1 Martín García, Jorge
A1 Fernández Fernández, María Mercedes
A1 Díez Casero, Julio Javier
K1 Biocontrol
K1 Análisis de imagen
K1 Micovirus
K1 Image analysis
K1 Mycoviruses
K1 2417.09 Fitopatología
AB Laccase enzymes (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) play a major role in the degradation of phenolic compounds such as lignin. They are common in fungi and have been suggested to participate in host colonization by pathogenic fungi. Putative mycoviruses have recently been isolated from the causal agent of pine pitch canker disease, Fusarium circinatum Nirenberg & O'Donell. In this study, the effects of single and double mycoviral infections on laccase activity, growth rate and pathogenicity were investigated in fourteen F. circinatum strains. Extracellular laccase activity was analyzed by the Bavendamm test, image processing and a spectrophotometric method. Mycelial growth, in vivo pathogenicity and seedling survival probability were also determined in Monterrey pine (Pinus radiata D. Don) seedlings. The findings showed that (i) mycelial growth of isolates from the same fungal population was homogeneous, (ii) the presence of mycovirus appears to increase the virulence of fungal isolates, (iii) co-infection (with two mycoviruses) caused cryptic effects in fungal isolates, and (iv) laccases embody a possible auxiliary tool in fungal infection. The prospects for biocontrol, the adaptive role of F. circinatum mycoviruses and the importance of laccase enzymes in host colonization are discussed.
SN 0885-5765
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/40891
UL http://uvadoc.uva.es/handle/10324/40891
LA eng
NO Physiological and Molecular Plant Pathology, 2016, Volume 94, Pages 8-15
NO Producción Científica
DS UVaDOC
RD 22-dic-2024