RT info:eu-repo/semantics/article
T1 Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure
A1 Salvo Comino, Coral
A1 González Gil, Alfonso
A1 Rodríguez Valentín, Javier
A1 García Hernández, Celia
A1 Martín Pedrosa, Fernando
A1 García Cabezón, Ana Cristina
A1 Rodríguez Méndez, María Luz
K1 Catechol
K1 Catecol
K1 Gold nanoparticles
K1 Nanopartículas de oro
K1 Laccases
K1 Lacasas
K1 Tyrosinase
K1 Tirosinasa
K1 2307 Química Física
K1 2303 Química Inorgánica
AB Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.
PB MDPI
SN 1424-8220
YR 2020
FD 2020
LK http://uvadoc.uva.es/handle/10324/40943
UL http://uvadoc.uva.es/handle/10324/40943
LA eng
NO Sensors, 2020, vol. 20, n. 7. 12 p.
NO Producción Científica
DS UVaDOC
RD 22-dic-2024