RT info:eu-repo/semantics/article
T1 Isolation and molecular characterization of two lectins from dwarf elder (Sambucus ebulus L.) blossoms related to the Sam n1 allergen
A1 Jiménez López, María del Pilar
A1 Cabrero Lobato, Patricia
A1 Basterrechea Elizgaray, José Ezequiel
A1 Tejero del Río, Jesús
A1 Córdoba Diaz, Damián
A1 Girbés Juan, Tomás
K1 Dwarf elder
K1 Saúco menor
K1 Lectin
K1 Lectina
K1 Ebulin
K1 Ebulina
K1 Ricin
K1 Ricina
AB Sambucus species contain a number of lectins with and without antiribosomal activity. Here, we show that dwarf elder (Sambucus ebulus L.) blossoms express two D-galactose-binding lectins that were isolated and purified by affinity chromatography and gel filtration. These proteins, which we named ebulin blo (A-B toxin) and SELblo (B-B lectin)—blo from blossoms—were subjected to molecular characterization and analysis by MALDI-TOF mass spectrometry and tryptic peptide fingerprinting. Both lectins share a high degree of amino acid sequence homology with Sambucus lectins related to the Sam n1 allergen. Ebulin blo, but not SELblo, was highly toxic by nasal instillation to mice. Overall, our results suggested that both lectins would belong to an allergen family exemplified by Sam n1 and could trigger allergy responses. Furthermore, they raise a concern about ebulin blo toxicity.
PB MDPI
SN 2072-6651
YR 2013
FD 2013
LK http://uvadoc.uva.es/handle/10324/40993
UL http://uvadoc.uva.es/handle/10324/40993
LA eng
NO Toxins, 2013, vol. 5, n. 10. p. 1767-1779
NO Producción Científica
DS UVaDOC
RD 17-jul-2024