RT info:eu-repo/semantics/article
T1 Elicitor-dependent expression of the ribosome-inactivating protein beetin is developmentally regulated
A1 Iglesias Álvarez, María del Rosario
A1 Pérez, Yolanda
A1 Citores González, Lucía
A1 Ferreras Rodríguez, José Miguel
A1 Méndez, Enrique
A1 Girbés Juan, Tomás
K1 Beet
K1 Remolacha
K1 Hydrogen peroxide
K1 Peróxido de hidrógeno
K1 Ribosome-inactivating proteins
K1 Proteínas inactivadoras de ribosomas
K1 Salicylic acid
K1 Acido salicílico
AB BE27 and BE29 are two forms of beetin, a virus-inducible type 1 ribosome-inactivating protein isolated from leaves of Beta vulgaris L. Western blot analysis revealed the presence of beetin forms in adult plants but not in germ or young plants, indicating that the expression of these proteins is developmentally regulated. While beetins are expressed only in adult plants, their transcripts are present through all stages of development. In addition, the treatment of B. vulgaris leaves with mediators of plant-acquired resistance such as salicylic acid and hydrogen peroxide promoted the expression of beetin by induction of its transcript, but only in adult plants. The plant expresses three mRNAs which differ only in their 3′ untranslated region. All these observations suggest a dual regulation of beetin expression, i.e. at the post-transcriptional and transcriptional levels. Additionally, total RNA isolated from leaves treated with hydrogen peroxide, which express high levels of active beetin, is not de-adenylated by endogenous beetin, nor in vitro by the addition of BE27, thus suggesting that sugar beet ribosomes are resistant to beetin.
PB Oxford University Press
SN 0022-0957
YR 2008
FD 2008
LK http://uvadoc.uva.es/handle/10324/40997
UL http://uvadoc.uva.es/handle/10324/40997
LA eng
NO Journal of Experimental Botany, 2008, vol. 59, n. 6. p. 1215-1223
NO Producción Científica
DS UVaDOC
RD 22-nov-2024