RT info:eu-repo/semantics/article
T1 Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.
A1 Girbés Juan, Tomás
A1 Barbieri, Luigi
A1 Ferreras Rodríguez, José Miguel
A1 Arias Vallejo, Francisco Javier
A1 Iglesias Álvarez, María del Rosario
A1 Alegre, Carlos
A1 Escarmis, Cristina
A1 Stirpe, Fiorenzo
K1 Ribosome-inactivating proteins
K1 Proteínas inactivadoras de ribosomas
K1 Escherichia coli
K1 Agrobacterium tumefaciens
AB The effects of 30 type 1 and of 2 (ricin and volkensin) type 2 ribosome-inactivating proteins (RIPs) on Escherichia coli and Agrobacterium tumefaciens cell-free translation systems were compared with the effects on a rabbit reticulocyte translation system. The depurinating activity of RIPs on E. coli ribosomes was also evaluated. Only six type 1 RIPs inhibited endogenous mRNA-directed translational activity of E. coli lysates, with submicromolar 50% inhibitory concentrations. Four RIPs had similar activities on poly(U)-directed phenylalanine polymerization by E. coli ribosomes, and three RIPs inhibited poly(U)-directed polyphenylalanine synthesis by A. tumefaciens ribosomes, with submicromolar 50% inhibitory concentrations.
PB American Society for Microbiology
SN 0021-9193
YR 1993
FD 1993
LK http://uvadoc.uva.es/handle/10324/40998
UL http://uvadoc.uva.es/handle/10324/40998
LA eng
NO Journal of Bacteriology, 1993, vol. 175, n. 20. p. 6721-6724
NO Producción Científica
DS UVaDOC
RD 22-nov-2024