RT info:eu-repo/semantics/article
T1 Molecular characterization and systemic induction of single-chain ribosome-inactivating proteins (RIPs) in sugar beet (Beta vulgaris) leaves
A1 Iglesias Álvarez, María del Rosario
A1 Pérez, Yolanda
A1 Torre, Carlos de la
A1 Ferreras Rodríguez, José Miguel
A1 Antolín, Pilar
A1 Jiménez López, María del Pilar
A1 Rojo, María Ángeles
A1 Méndez, Enrique
A1 Girbés Juan, Tomás
K1 Protein synthesis inhibitor
K1 Inhibidor de síntesis proteica
K1 Ribosome-inactivating proteins
K1 Proteínas inactivadoras de ribosomas
K1 Sugar beet
K1 Remolacha azucarera
AB Sugar beet (Beta vulgaris L.) leaves contain virusinducible type 1 (single chain) ribosome-inactivatingproteins that have been named beetins. The structuraland functional characterization, the cellular location,and the potential role of beetins as antiviral agents arereported here. Beetins are formed of a single polypeptide chain with a varying degree of glycosylationand strongly inhibited in vitro protein synthesis inrabbit reticulocyte lysates (IC5051.15 ng ml21) anda Vicia sativa L. cell-free system (IC50568 ng ml21)through the single depurination of the large rRNA.Beetins trigger the multidepurination of tobacco mosaic virus (TMV) genomic RNA which underwent extensive degradation upon treatment with acid aniline.Beetins are extracellular proteins that were recoveredfrom the apoplastic fluid. Induction of sugar beet RIPswith either H2O2 or artichoke mottled crinkle virus(AMCV) was observed in leaves distant from the siteof application of such elicitors. The external application of purified beetin to sugar leaves prevented infection by AMCV which supports the preliminaryhypothesis that beetins could be involved in plantsystemic acquired resistance subjected to inductionby phytopathogens.
PB Oxford University Press
SN 0022-0957
YR 2005
FD 2005
LK http://uvadoc.uva.es/handle/10324/41031
UL http://uvadoc.uva.es/handle/10324/41031
LA eng
NO Journal of Experimental Botany, 2005, vol. 56, n. 416. p. 1675-1684
NO Producción Científica
DS UVaDOC
RD 27-dic-2024