RT info:eu-repo/semantics/bachelorThesis
T1 Inmovilización de aminoacilasas de streptomyces ambofaciens
A1 Herreros Hortelano, Sara
A2 Universidad de Valladolid. Escuela de Ingenierías Industriales
K1 Aminoacilasa
K1 Inmovilización
K1 Óxido de grafeno
K1 Cobalto
K1 2302.09 Enzimología
AB Enzymes are widely used as biocatalysts for different reactions. When operating with free-formenzymes they are in a liquid state so it will be difficult to separate them from the reactionproduct if complex methods are not used. If the enzyme is not fully recovered, the product willbe contaminated. Besides, by using the enzyme in this way, it cannot be reused in otherreactions and the stability time of the enzyme for the operation is low. These problems could besolved by immobilizing the enzymes since they are more stables when fixed to a carrier and sincethey are in a solid form they are better handled, easier to separate from the product and can bereused. Despite the advantages that the immobilization can have, when fixing the enzymes to acarrier they can lose activity. That is why is necessary to choose the support correctly and theimmobilization method for the fixation to be effective.In the laboratory aminoacylases are produced from Streptomyces ambofaciens; however, it isnecessary to immobilize them because as it is been said, in their free form they are very unstable.The objectives of the present work is to be able to compare the immobilizations of theseenzymes, the aminoacylases, in different supports, silicon or carbonated. In addition, optimalconditions have to be found to carry out the immobilization step, both in terms of temperatureand pH, the buffer used and the support in which the immobilization is carried out
YR 2020
FD 2020
LK http://uvadoc.uva.es/handle/10324/41776
UL http://uvadoc.uva.es/handle/10324/41776
LA eng
NO Departamento de Ingeniería Química y Tecnología del Medio Ambiente
DS UVaDOC
RD 12-abr-2025