RT info:eu-repo/semantics/article
T1 Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients
A1 Vallejo Diez, Sara
A1 Bernardo Ordiz, David
A1 Moreno, María de Lourdes
A1 Muñoz Suano, Alba
A1 Fernández Salazar, Luis Ignacio
A1 Calvo, Carmen
A1 Sousa, Carolina
A1 Garrote Adrados, José Antonio
A1 Cebolla, Ángel
A1 Arranz Sanz, Eduardo
K1 Anticuerpos
K1 Enfermedad celíaca
K1 3205 Medicina Interna
AB We studied whether celiac disease (CD) patients produce antibodies against a novel gliadin peptide specifically generated in the duodenum of CD patients by a previously described pattern of CD-specific duodenal proteases. Fingerprinting and ion-trap mass spectrometry of CD-specific duodenal gliadin-degrading protease pattern revealed a new 8-mer gliadin-derived peptide. An ELISA against synthetic deamidated 8-mer peptides (DGP 8-mer) was used to study the presence of IgA anti-DGP 8-mer antibodies in plasma samples from 81 children (31 active CD patients (aCD), 17 CD patients on a gluten-free diet (GFD), 10 healthy controls (C) and 23 patients with other gastrointestinal pathology (GP)) and 101 adults (16 aCD, 12 GFD, 27 C and 46 GP-patients). Deamidation of the 8-mer peptide significantly increased the reactivity of the IgA antibodies from CD patients against the peptide. Significant IgA anti-DGP 8-mer antibodies levels were detected in 93.5% of aCD-, 11.8% of GFD- and 4.3% of GP-patients in children. In adults, antibodies were detected in 81.3% of aCD-patients and 8.3% of GFD-patients while were absent in 100% of C- and GP-patients. Duodenal CD-specific gliadin degrading proteases release an 8-mer gliadin peptide that once deamidated is an antigen for specific IgA antibodies in CD patients which may provide a new accurate diagnostic tool in CD.
PB Public Library of Science
SN 1932-6203
YR 2013
FD 2013
LK http://uvadoc.uva.es/handle/10324/41885
UL http://uvadoc.uva.es/handle/10324/41885
LA eng
NO Plos One, 2013, vol. 8, n. 11
NO Producción Científica
DS UVaDOC
RD 25-nov-2024