RT info:eu-repo/semantics/article
T1 Ascorbate-dependent decrease of the mucosal immune inflammatory response to gliadin in coeliac disease patients
A1 Bernardo Ordiz, David
A1 Martínez Abad, Beatriz
A1 Vallejo Diez, Sara
A1 Montalvillo Álvarez, Enrique
A1 Benito, V.
A1 Anta, B.
A1 Fernández Salazar, Luis Ignacio
A1 Garrote Adrados, José Antonio
K1 Enfermedad celíaca
AB Background: The IL-15/NF- B axis has an important role in coeliac disease (CD) and may representa molecular target for immunomodulation. Ascorbate (vitamin C) is known to showinhibitory effects on NF- B. Therefore, we studied if ascorbate supplementation to gliadingliadin-stimulated biopsy culture could down-regulate the mucosal immune response to gliadinin CD.Methods: Duodenal biopsy explants from treated CD patients were gliadin challenged in vitro(100  g/ml) with and without 20 mM ascorbate. An extra tissue explant in basal culture wasused as internal control. Secretion levels of nitrites (3 h), and IFN , TNF , IFN , IL-17, IL-13,and IL-6 (24 h) were measured on the supernatants. IL-15 was assayed by western-blot on wholeprotein duodenal explants.Results: The addition of ascorbate to in vitro culture gliadin-challenged biopsies blocked thesecretion of nitrites (p = 0.013), IFN  (p = 0.0207), TNF  (p = 0.0099), IFN  (p = 0.0375), andIL-6 (p = 0.0036) compared to samples from non-ascorbate supplemented culture. Cytokinesecretion was downregulated by ascorbate even to lower values than those observed in basalcultures (IFN : p = 0.0312; TNF : p = 0.0312; IFN : p = 0.0312; and IL-6: p = 0.0078). Gliadinchallengeinduced IL-15 production in biopsies from treated CD patients, while the additionof ascorbate to culture medium completely inhibited IL-15 production. Moreover, the inhibitionof IL-15 by ascorbate took place even in the only treated CD-patient who had basal IL-15production.Conclusions: Ascorbate decreases the mucosal inflammatory response to gluten in an intestinalbiopsy culture model, so it might have a role in future supplementary therapy in CD.© 2010 SEICAP. Published by Elsevier España, S.L. All rights reserve
PB Elsevier
SN 0301-0546
YR 2012
FD 2012
LK http://uvadoc.uva.es/handle/10324/4201
UL http://uvadoc.uva.es/handle/10324/4201
LA eng
NO Allergol. Immunopathol. (Madr.) 2012, vol. 40, n. 1. p.3-8
NO Producción Científica
DS UVaDOC
RD 22-nov-2024