RT info:eu-repo/semantics/article
T1 Elastin-like recombinamers in collagen-based tubular gels improve cell-mediated remodeling and viscoelastic properties
A1 Camasão, Dimitria Bonizol
A1 González Pérez, Miguel
A1 Palladino, Sara
A1 Alonso Rodrigo, Matilde
A1 Rodríguez Cabello, José Carlos
A1 Mantovani, Diego
AB Natural polymers are commonly used as scaffolds for vascular tissue engineering. The recognized biological properties of this class of materials are often counterbalanced by their low mechanical performance. In this work, recombinant elastin-like polypeptides (or elastin-like recombinamers, ELRs) weremixed with collagen gel and cells to produce cellularized tubular constructs in an attempt to recapitulatethe mechanical behavior of the vascular extracellular matrix (ECM). The presence of the elastic proteininfluenced cell-mediated remodeling evaluated in terms of construct compaction, cell proliferation andECM (collagen, elastin and fibrillin-1) gene expression. The partial substitution of collagen with ELR andthe observed differences in cellular behavior synergistically contributed to the superior viscoelastic properties of the constructs containing 30% ELR and 70% of collagen (in mass). This led to the improvementof 40% in the initial elastic modulus, 50% in the equilibrium elastic modulus, and 37% in the tensilestrength at break without compromising the strain at break, when compared to a pure collagen scaffold.Suggestions for future research include modifications in the crosslinking technology, ELR composition,polymer concentration, cell seeding density and dynamic stimulation, which have the potential to furtherimprove the mechanical performance of the constructs towards physiological values.
PB The Royal Society of Chemistry
SN 2047-4830
YR 2020
FD 2020
LK http://uvadoc.uva.es/handle/10324/42416
UL http://uvadoc.uva.es/handle/10324/42416
LA spa
NO Biomaterials  Science. Julio 2020, vol 8, p. 3536–3548
NO Producción Científica
DS UVaDOC
RD 21-dic-2024