RT info:eu-repo/semantics/article
T1 Expression of MicroRNA-15b and the glycosyltransferase GCNT3 correlates with antitumor efficacy of rosemary diterpenes in colon and pancreatic cancer
A1 González-Vallinas Garrachón, Margarita
A1 Molina, Susana
A1 Vicente, Gonzalo
A1 Zarza, Virginia
A1 Martín Hernández, Roberto
A1 García Risco, Mónica R.
A1 Fornari, Tiziana
A1 Reglero, Guillermo
A1 Ramírez de Molina, Ana
K1 Cáncer de colon
K1 Cáncer de páncreas
K1 Romero
K1 3207.13 Oncología
AB Colorectal and pancreatic cancers remain important contributors to cancer mortality burden and, therefore, newtherapeutic approaches are urgently needed. Rosemary (Rosmarinus officinalis L.) extracts and its components have beenreported as natural potent antiproliferative agents against cancer cells. However, to potentially apply rosemary as acomplementary approach for cancer therapy, additional information regarding the most effective composition, itsantitumor effect in vivo and its main molecular mediators is still needed. In this work, five carnosic acid-rich supercriticalrosemary extracts with different chemical compositions have been assayed for their antitumor activity both in vivo (in nudemice) and in vitro against colon and pancreatic cancer cells. We found that the antitumor effect of carnosic acid togetherwith carnosol was higher than the sum of their effects separately, which supports the use of the rosemary extract as awhole. In addition, gene and microRNA expression analyses have been performed to ascertain its antitumor mechanism,revealing that up-regulation of the metabolic-related gene GCNT3 and down-regulation of its potential epigeneticmodulator miR-15b correlate with the antitumor effect of rosemary. Moreover, plasmatic miR-15b down-regulation wasdetected after in vivo treatment with rosemary. Our results support the use of carnosic acid-rich rosemary extract as acomplementary approach in colon and pancreatic cancer and indicate that GCNT3 expression may be involved in itsantitumor mechanism and that miR-15b might be used as a non-invasive biomarker to monitor rosemary anticancer effect.
PB Public Library of Science
SN 1932-6203
YR 2014
FD 2014
LK http://uvadoc.uva.es/handle/10324/42590
UL http://uvadoc.uva.es/handle/10324/42590
LA eng
NO PLoS ONE, 2014, vol 9, n. 6,  e98556
NO Producción Científica
DS UVaDOC
RD 25-nov-2024