RT info:eu-repo/semantics/article
T1 Occurrence of hepatitis E virus in pigs and pork cuts and organs at the time of slaughter, spain, 2017
A1 García, Nerea
A1 Hernández, Marta
A1 Gutierrez Boada, Maialen
A1 Valero, Antonio
A1 Navarro, Alejandro
A1 Muñoz Chimeno, Milagros
A1 Fernández Manzano, Alvaro
A1 Escobar, Franco Matías
A1 Martínez, Irene
A1 Bárcena, Carmen
A1 González, Sergio
A1 Avellón, Ana
A1 Eiros Bouza, José María
A1 Fongaro, Gislaine
A1 Domínguez, Lucas
A1 Goyache, Joaquín
A1 Rodríguez Lázaro, David
K1 Virus de la hepatitis E
K1 Seroprevalencia
K1 Seguridad alimentaria
K1 2420 Virología
AB Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is afoodborne disease that has emerged in Europe in recent decades. The main animalreservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are consideredthe major risk products, and studies focused on the presence of HEV in pig musclesare scarce. The objective of the present study was to evaluate the presence ofHEV in different organs and tissues of 45 apparently healthy pigs from nine Spanishslaughterhouses (50% national production) that could enter into the food supply chain.Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples fromeach animal were analyzed for the presence of HEV RNA by reverse transcription realtimePCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). Fromthe 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified inthe liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm(1/45). This is the first report on detection of HEV RNA in kidney and heart samples ofnaturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, andloin samples. These findings indicate that pig meat could be considered as a low riskmaterial for foodborne HEV infection.
PB Frontiers Media
SN 1664-302X
YR 2020
FD 2020
LK http://uvadoc.uva.es/handle/10324/42924
UL http://uvadoc.uva.es/handle/10324/42924
LA eng
NO Frontiers in Microbiology, 2020, vol. 10, art. 2990
NO Producción Científica
DS UVaDOC
RD 17-jul-2024