RT info:eu-repo/semantics/article
T1 Interleukin 18 maintains a long-standing inflammation in coeliac disease patients
A1 León, Alberto José
A1 Garrote Adrados, José Antonio
A1 Calvo Romero, María del Carmen
A1 Fernández Salazar, Luis Ignacio
A1 Villar, A. del
K1 Enfermedad celíaca
K1 Citokinas
AB Dietary gluten induces an early response in the intestine of coeliac diseasepatients (CD), within a few hours, and this is driven by high levels of proinflammatorycytokines, including IFNg and IL-15, as has been thoroughlyshown by gluten stimulation of biopsy explants. Our aim was to identify theimmune mediators involved in the long-standing inflammation in untreatedCD patients at diagnosis. mRNA and protein levels of TNFa, IL-12(p35),IL-12(p40), IL-15, IL-18 and IL-23(p19) were quantified in biopsies fromactive CD patients, CD patients on a gluten-free diet (GFD), healthy controls,and patients with non-CD inflammation and mild histological changes in theintestine. Biopsies from CD patients on a GFD were also stimulated in vitrowith gliadin, and protein expression of IL-15 and IL-18 was analysed. Levels ofIL-12 and IL-23 mRNA are nearly absent, and TNFa levels remain unchangedamong different groups. Both the active and inactive forms of IL-18 proteinhave been found in all samples from active CD, and protein expression wasonly localized within the crypts. Levels of IL-15 mRNA remain unchanged,and protein expression, localized within the lamina propria, is found in asmall number of samples. In vitro stimulation with gluten induces the expressionof IL-15 and IL-18. In active CD, the early response following glutenintake characterized by high IFNg levels is driven by IL-18, and probablyIL-15, and this alternates with periods of long-standing inflammation withmoderate IFNg levels, maintained by IL-18 alone.
PB British Society for Immunology
YR 2006
FD 2006
LK http://uvadoc.uva.es/handle/10324/4329
UL http://uvadoc.uva.es/handle/10324/4329
LA eng
NO Clinical and Experimental Immunology, vol.146, p. 479-485
NO Producción Científica
DS UVaDOC
RD 22-nov-2024