RT info:eu-repo/semantics/article
T1 Chondroitin sulphate proteoglycan and embryonic brain inlargement in the chick
A1 Gato Casado, Ángel Luis
A1 Moro Balbás, José Antonio
A1 Pastor Vázquez, Juan Francisco
A1 Represa de la Guerra, Juan
A1 Barbosa Ayucar, Enrique
K1 Fluido intraneural - Composición
AB Abstraer. Prcvious studies  of thc car l y dcvclopment of the ncural  tube  ha ve shown  thc ex istence of an  intraneural nuid,  which  causes  a  positivc   pressure  inside  this  pri­ m ordium, and seems to play a key role in the early dcvcl­ opmcn l  of  the  central  nervous syslc m.  I n  the  present sludy  wc invest igated  the composition and  synthesis of lhis   int ra neural   fluid.  By  usi n g  a  scqucntial  method, wh ich     includes    fixation    with    gl utaraldch yde    plus cclylpyridinium chl oride, opening thc ncural  cavity after critica!  point  drying  and  scan ning clcctron   microscopy analysis, we found  a  water-soluble extracellular matrix that  filled up  thc brain  vesicles of chick  embryos al  the ea rliest stages of thc ncural  tu be. An ultrastructura l study of the neural  cpit hcliu m during these stages  revea led  the cxistcnce of a secretion proccss in the neural  cells toward thc apical side, the future neural cavity. An immunocyto­ chemical  study  to asscs thc nature  of the secreted  materi­ al has shown  that  the intraneu ral matrix  conta ins chon­ droitin  sulphate proteoglycan, which appeared homoge­ n ously  distributed  throughout  lhc  neura l  cavity.  Our findings demonstra te that  thc int raneural liquid is a nuid of com plcx composilion and  includes  chondroiti n su l­ phate   protcoglycan as  a n  osmotically active  molccule. Th is suggests  a morphogenetic role for the proteoglycan during ea rl y brain enlargcmcnt. Thc neural ectoderm is a polarized   epi thelium   from  carly  developmental  stages and  secretes  the in t rancural  malrix.
PB Springer-Verlag
SN 0340-2061
YR 1993
FD 1993
LK http://uvadoc.uva.es/handle/10324/4443
UL http://uvadoc.uva.es/handle/10324/4443
LA spa
NO Anat. Embryol.
NO Producción Científica
DS UVaDOC
RD 22-dic-2024