RT info:eu-repo/semantics/article
T1 Phenotypic modulation of cultured primary human aortic vascular smooth muscle cells by uremic serum
A1 Cazaña Pérez, Violeta
A1 Cidad Velasco, María del Pilar
A1 Donate Correa, Javier
A1 Martín Núñez, Ernesto
A1 López López, José Ramón
A1 Pérez García, María Teresa
A1 Giráldez Fernández, Teresa
A1 Navarro González, Juan Francisco
A1 Álvarez de la Rosa, Diego
K1 Vascular calcification
K1 Calcificación vascular
K1 Uremia
K1 Chronic kidney disease
K1 Enfermedad renal crónica
K1 Apoptosis
K1 Human aorta
K1 Arteria aorta
AB Patients with chronic kidney disease (CKD) have a markedly increased incidence of cardiovascular disease (CVD). The high concentration of circulating uremic toxins and alterations in mineral metabolism and hormone levels produce vascular wall remodeling and significant vascular damage. Medial calcification is an early vascular event in CKD patients and is associated to apoptosis or necrosis and trans-differentiation of vascular smooth muscle cells (VSMC) to an osteogenic phenotype. VSMC obtained from bovine or rat aorta and cultured in the presence of increased inorganic phosphate (Pi) have been extensively used to study these processes. In this study we used human aortic VSMC primary cultures to compare the effects of increased Pi to treatment with serum obtained from uremic patients. Uremic serum induced calcification, trans-differentiation and phenotypic remodeling even with normal Pi levels. In spite of similar calcification kinetics, there were fundamental differences in osteochondrogenic marker expression and alkaline phosphatase induction between Pi and uremic serum-treated cells. Moreover, high Pi induced a dramatic decrease in cell viability, while uremic serum preserved it. In summary, our data suggests that primary cultures of human VSMC treated with serum from uremic patients provides a more informative model for the study of vascular calcification secondary to CKD.
PB Frontiers
SN 1664-042X
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/44479
UL http://uvadoc.uva.es/handle/10324/44479
LA eng
NO Frontiers in Physiology, 2018, vol 9, n. 89. 14 p.
NO Producción Científica
DS UVaDOC
RD 28-nov-2024