RT info:eu-repo/semantics/article
T1 Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells
A1 Cidad Velasco, María del Pilar
A1 Moreno Domínguez, Alejandro
A1 Novensá, Laura
A1 Roqué, Mercé
A1 Barquín, Leire
A1 Heras i Fortuny, Maria Magdalena
A1 Pérez García, María Teresa
A1 López López, José Ramón
K1 Gene expression
K1 Expresión génica
K1 Ion channels
K1 Canales iónicos
K1 Restenosis
K1 Vascular smooth muscle
K1 Músculo liso vascular
AB Objective: Vascular smooth muscle cells (VSMCs) contribute significantly to occlusive vascular diseases by virtue of their ability to switch to a noncontractile, migratory, and proliferating phenotype. Although the participation of ion channels in this phenotypic modulation (PM) has been described previously, changes in their expression are poorly defined because of their large molecular diversity. We obtained a global portrait of ion channel expression in contractile versus proliferating mouse femoral artery VSMCs, and explored the functional contribution to the PM of the most relevant changes that we observed.Methods and Results: High-throughput real-time polymerase chain reaction of 87 ion channel genes was performed in 2 experimental paradigms: an in vivo model of endoluminal lesion and an in vitro model of cultured VSMCs obtained from explants. mRNA expression changes showed a good correlation between the 2 proliferative models, with only 2 genes, Kv1.3 and Kvβ2, increasing their expression on proliferation. The functional characterization demonstrates that Kv1.3 currents increased in proliferating VSMC and that their selective blockade inhibits migration and proliferation.Conclusion: These findings establish the involvement of Kv1.3 channels in the PM of VSMCs, providing a new therapeutical target for the treatment of intimal hyperplasia.
PB American Heart Association
SN 1524-4636
YR 2010
FD 2010
LK http://uvadoc.uva.es/handle/10324/44599
UL http://uvadoc.uva.es/handle/10324/44599
LA eng
NO Arteriosclerosis, Thrombosis, and Vascular Biology, 2010, vol. 30, n. 6. p. 1203-1211
NO Producción Científica
DS UVaDOC
RD 27-jul-2024