RT info:eu-repo/semantics/article
T1 Transcriptomic analysis of calcium remodeling in colorectal cancer
A1 Pérez Riesgo, Enrique
A1 González Gutiérrez, Lucía
A1 Ubierna, Daniel
A1 Acedo, Alberto
A1 Moyer, Mary P.
A1 Núñez Llorente, Lucía
A1 Villalobos Jorge, Carlos
K1 Colorectal cancer
K1 Cáncer colorrectal
K1 Calcium
K1 Calcio
K1 Transcriptomics
K1 Transcriptoma
K1 RNA-sequencing
K1 Secuenciación de ARN
AB Colorectal cancer (CRC) cells undergo the remodeling of intracellular Ca2+ homeostasis, which contributes to cancer hallmarks such as enhanced proliferation, invasion and survival. Ca2+ remodeling includes critical changes in store-operated Ca2+ entry (SOCE) and Ca2+ store content. Some changes have been investigated at the molecular level. However, since nearly 100 genes are involved in intracellular Ca2+ transport, a comprehensive view of Ca2+ remodeling in CRC is lacking. We have used Next Generation Sequencing (NGS) to investigate differences in expression of 77 selected gene transcripts involved in intracellular Ca2+ transport in CRC. To this end, mRNA from normal human colonic NCM460 cells and human colon cancer HT29 cells was isolated and used as a template for transcriptomic sequencing and expression analysis using Ion Torrent technology. After data transformation and filtering, exploratory analysis revealed that both cell types were well segregated. In addition, differential gene expression using R and bioconductor packages show significant differences in expression of selected voltage-operated Ca2+ channels and store-operated Ca2+ entry players, transient receptor potential (TRP) channels, Ca2+ release channels, Ca2+ pumps, Na+/Ca2+ exchanger isoforms and genes involved in mitochondrial Ca2+ transport. These data provide the first comprehensive transcriptomic analysis of Ca2+ remodeling in CRC.
PB MDPI
SN 1422-0067
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/45031
UL http://uvadoc.uva.es/handle/10324/45031
LA eng
NO International Journal of Molecular Sciences, 2017, vol. 18, n. 5. 23 p.
NO Producción Científica
DS UVaDOC
RD 22-nov-2024